Antioxidative activity of glycoprotein isolated from Geranium sibiricum Linne

Abstract

Glycoprotein from Geranium sibiricum Linne (GSL) with 18 kDa was isolated and it consists of carbohydrate moiety (10.45%) and protein moiety (89.55%). The GSL glycoprotein was characterised by its radical scavenging activity under various experimental conditions. When GSL glycoprotein was treated with deactivation agents (pronase E or NaIO₄), its scavenging activity decreased in both cases. It has optimal and maximal activity in acidic, neutral pH (up to pH 9), and up to 85°C. Also, its activity reduced in the case of Ca²⁺ and Mn²⁺, with the exception of the Mg²⁺ case. Its activity in the presence of Mn²⁺ declined more than in the case of the Ca²⁺. Also, GSL glycoprotein (500 µg mL⁻¹) has antioxidative effects on hydroxyl and superoxide anion radicals in cell-free systems, and GSL glycoprotein (200 µg mL⁻¹) significantly protected from cytotoxicity in the GO (100 mU mL⁻¹)-treated Chang liver cells for 4 h.

Keywords:

• Geranium sibiricum Linne (GSL) glycoprotein
• antioxidative activity
• reactive oxygen radicals

Acknowledgements

This study was financially supported by Chonnam National University in 2007.