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Natural Product Research
Formerly Natural Product Letters
Volume 24, 2010 - Issue 19
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Original Articles

Essential oil composition of leaves of Stachys yemenensis obtained by supercritical CO2

Nasser A. Awadh Ali Department of Pharmacognosy, Sana’a University, Sana’a, Yemen
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Bruno Marongiu Dipartimento di Scienze Chimiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SS 554, Km 4,500, 09042 Cagliari, ItalyCorrespondence[email protected]
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Alessandra Piras Dipartimento di Scienze Chimiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SS 554, Km 4,500, 09042 Cagliari, Italy
,
Silvia Porcedda Dipartimento di Scienze Chimiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SS 554, Km 4,500, 09042 Cagliari, Italy
,
Danilo Falconieri Dipartimento di Scienze Chimiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SS 554, Km 4,500, 09042 Cagliari, Italy
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Paola Molicotti Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università di Sassari, Viale San Pietro 47b, 07100 Sassari, Italy
&
Stefania Zanetti Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università di Sassari, Viale San Pietro 47b, 07100 Sassari, Italy
 show all
Pages 1823-1829 | Received 20 Nov 2009, Accepted 16 Feb 2010, Published online: 19 Nov 2010
 
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Abstract

This article reports the composition of the essential oil from the leaves of Stachys yemenensis. The essential oil was extracted by supercritical CO2 (90 bar; 40°C) and its chemical composition was determined by gas chromatography and gas chromatography–mass spectrometry. The major components of the sample were α-phellandrene (13.9%), β-phellandrene (11.7%), elemol (12.0%), spathulenol (6.7%), β-eudesmol (5.0%), α-eudesmol (4.75%) and squalene (4.8%). On the exhausted matrix, deprived of the volatiles, we carried out a high-pressure (250 bar) treatment for the extraction of squalene (49.7%). The antimicrobial activity of the essential oils has been assayed by using the broth dilution method on two American Type Culture Collection (ATCC) strains, Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 43300, and two clinical strains, Candida albicans and Candida glabrata.

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