In vitro cytotoxic and antiviral activities of Ficus carica latex extracts

Abstract

The latex of fig fruit (Ficus carica) is used in traditional medicine for the treatment of skin infections such as warts and also diseases of possible viral origin. Five extracts (methanolic, hexanic, ethyl acetate, hexane–ethyl acetate (v/v) and chloroformic) of this species were investigated in vitro for their antiviral potential activity against herpes simplex type 1 (HSV-1), echovirus type 11 (ECV-11) and adenovirus (ADV). To evaluate the capacity of the extracts to inhibit the replication of viruses, the following assays were performed: adsorption and penetration, intracellular inhibition and virucidal activity. Observation of cytopathic effects was used to determine the antiviral action. The hexanic and hexane–ethyl acetate (v/v) extracts inhibited multiplication of viruses by tested techniques at concentrations of 78 µg mL⁻¹. These two extracts were possible candidates as herbal medicines for herpes virus, echovirus and adenovirus infectious diseases. All extracts had no cytotoxic effect on Vero cells at all tested concentrations.

Keywords:

• Ficus carica
• latex
• herpes simplex 1
• echovirus 11
• adenovirus
• antiviral activity

Acknowledgement

The authors thank Professor Ben Ouada Hafed, Directeur de l’Institut Supérieur des Sciences Appliquées et de Technologie de MAHDIA.