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Natural Product Research
Formerly Natural Product Letters
Volume 27, 2013 - Issue 4-5: Special Issue in Honour of Professor Atta-Ur-Rahman
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Research Articles

Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb ‘Kwao Keur’ (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol

Satoko Shimokawa Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan
,
Takuya Kumamoto Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan
,
Tsutomu Ishikawa Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, JapanCorrespondence[email protected]
,
Miho Takashi Research and Development, Shiratori Pharmaceutical Co. Ltd., 6-11-24 Tsudanuma, Narashino, Chiba 275-0016, Japan
,
Yoshihiro Higuchi Research and Development, Shiratori Pharmaceutical Co. Ltd., 6-11-24 Tsudanuma, Narashino, Chiba 275-0016, Japan
,
Chaiyo Chaichantipyuth Faculty of Pharmaceutical Sciences, Chulalongkorn University, Patchai, Bangkok 10330, Thailand
&
Sunee Chansakaow Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
 show all
Pages 371-378 | Received 09 Mar 2012, Accepted 10 Apr 2012, Published online: 18 Jun 2012
 
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Abstract

Quantitative analysis of miroestrol (1) and kwakhurin (3) by HPLC, leading to standardisation of commercially available Thai miracle herb ‘Kwao Keur’ which has been identified with Pueraria mirifica, was established using independent solvent systems. The simple isolation procedure of highly estrogenic miroestrol (1) and deoxymiroestrol (2) from P. mirifica was also proposed.

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