Abstract
A combinative method of HPLC fingerprinting and quantitative determination was successfully applied to monitor dynamic accumulation of ginsenosides in five-year-old Panax ginseng roots from different harvest times. The optimal chromatographic conditions were achieved on a C18 column with gradient elution using acetonitrile and 1 mmol · L−1 KH2PO4 buffer solution at 203 nm. The result indicated that the contents of total ginsenosides showed significant variations, and a decrease tendency appeared in the growth period of the fifth year.
Acknowledgements
This research work was financially supported by the Key Project of National Natural Science Foundation of China (30830116), and a Project Funded by The Priority Academic Program Development of Jiangsu Higher Education Institutions.