Chemical constituents and biological activities from roots of Enkleia siamensis

Abstract

Chemical investigation from roots of Enkleia siamensis (Kurz) Nervling resulted in the isolation of 10 compounds. Their structures were established on the basis of 1D and 2D NMR spectroscopic data as linobiflavonoid (1), chamaejasmin (2), 7-O-β-d-glucopyranosylchamaejasmin (3), ormocarpin (4), ( − )-wikstromol (5), matairesinol (6), (+)-lariciresinol (7), umbelliferone (8), daphnoretin (9) and carthamidin (10). Compounds 2 and 8 showed cytotoxicity against KB, MCF-7 and NCI-H187 cancer cell lines. Compounds 1, 2 and 5 showed weak minimum inhibitory requirements to acetylcholinesterase with values ranging from 50 to 1000 ng. In addition, compound 2 exhibited antimalarial activity against Plasmodium falciparum with an IC₅₀ value of 2.32 μg/mL.

Keywords::

• Enkleia siamensis
• Thymelaeaceae
• antimalarial
• cytotoxicity
• acetylcholinesterase

Acknowledgements

Financial support came from the Thailand Research Fund via the Royal Golden Jubilee Ph.D. programme (0353/2551). We thank the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Advanced Functional Materials Cluster of Khon Kaen University and the Center of Excellence for Innovation in Chemistry (PERCH-CIC) for partial support. We are indebted to the Bioassay Research Facility of the National Center for Genetic Engineering and Biotechnology via the Bioresources Research Network (BRN) for bioactivity tests.

Notes

A part of this work was presented as a poster at Pure and Applied Chemistry International Conference (PACCON2013), the Tide Resort, Bangsaen Beach, Chonburi Thailand, on 23–25 January 2013.