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Natural Product Research
Formerly Natural Product Letters
Volume 28, 2014 - Issue 16
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Short Communication

Antioxidant and antifungal activities of Smilax campestris Griseb. (Smilacaceae)

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Pages 1275-1279 | Received 27 Dec 2013, Accepted 07 Feb 2014, Published online: 07 Apr 2014
 

Abstract

Ethanol extract and fractions obtained from aerial parts of Smilax campestris were examined in order to determine their phenolic composition, antioxidant capacity and antifungal activities. High-performance liquid chromatography coupled with DAD analysis indicated that quercetin and rutin were the main phenolic compounds present in butanol fraction and ethanol extract, respectively. The antioxidant activity assessed by the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radical was significantly more pronounced for the ethanol extract and butanol fraction than that of the commercial antioxidant 2,6-di-tert-butyl-4-methylphenol. The antifungal activity of extract and fractions was investigated by using microdilution method against five Candida and two Cryptococcus yeast strains. Ethanol extract and fractions exhibited antifungal activities against Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus gattii. This work provides the knowledge of profile and content of flavonoids and their antioxidant and antifungal activities in the extract and fractions of aerial parts of S. campestris.

Acknowledgements

We thank Prof. Dr Douglas Antônio de Carvalho for botanical identification of this plant.

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