Biochemical and radical-scavenging properties of sea cucumber (Stichopus vastus) collagen hydrolysates

Abstract

The molecular mass distribution, amino acid composition and radical-scavenging activity of collagen hydrolysates prepared from collagen isolated from the sea cucumber Stichopus vastus were investigated. β and α₁ chains of the collagen were successfully hydrolysed by trypsin. The molecular mass distribution of the hydrolysates ranged from 5 to 25 kDa, and they were rich in glycine, alanine, glutamate, proline and hydroxyproline residues. The hydrolysates exhibited excellent radical-scavenging activity. These results indicate that collagen hydrolysates from S. vastus can be used as a functional ingredient in food and nutraceutical products.

Keywords::

• sea cucumber
• integument collagen
• trypsin hydrolysis
• collagen hydrolysates
• biochemical and radical scavenging properties

Acknowledgements

Authors would like to thank Universiti Sains Malaysia for providing financial support through USM Fellowship and ERGS (203/CAATS/6730103) grant.