Influence of extraction solvent on antioxidant capacity value of oleaster measured by ORAC method

Abstract

Oxygen radical absorbance capacity (ORAC) is a widely used hydrogen atom transfer-based method which measures the antioxidant capacity of natural products. ORAC values of oleaster (Elaeagnus angustifolia L.), which was extracted with ethanol/acetone (7:3, v/v), ethanol/water (1:1, v/v) and methanol/water (1:1, v/v) in order to evaluate the effects of solvent type on antioxidant capacity, were examined. In general, results revealed that ethanol/water extracts exhibited better antioxidant capacity values. Furthermore, results obtained by using ORAC-eosin y (ORAC-EY), one of the widely used derivative of fluorescein (FL), as a fluorescent probe were compared with those obtained by using ORAC-FL. According to the results, ORAC-EY values were found to be compatible with ORAC-FL values.

Keywords::

• Elaeagnus angustifolia L.
• antioxidant capacity
• ORAC

Acknowledgements

This research was supported by the Ege University Research Fund, Scientific Research Foundation (BAP). The authors wish to thank the Ege University Faculty of Pharmacy Pharmaceutical Sciences Research Centre (FABAL).