Simultaneous determination of six constituents in Mahuang Fuzi Xixin by UPLC-PDA–MS/MS

Abstract

Mahuang Fuzi Xixin (MFX), a classic recipe in traditional Chinese medicine, belongs to an exterior-relieving formula. For quality control of the MFX products, qualitative analysis using ultra-high performance liquid chromatography with diode-array detector–tandem mass spectrometry (UPLC-PDA–MS/MS) was undertaken. Six compounds from the MFX were simultaneously detected. Among them, astragalin and kaempferol 3-rutinoside were quantified through the UPLC–MS/MS method, while asarinin, sesamin, kakuol and methyleugenol were quantified through the UPLC-PDA method. This method can be applied to the quantitative determination of the six compounds in the MFX.

Keywords::

• Mahuang Fuzi Xixin
• simultaneous determination
• UPLC-PDA–MS/MS

Additional information

Funding

This work was supported by Special fund for post-doctoral innovation project of Shandong province [grant number 201203009], Innovative Drug Incubation Base Project from Liaoning Province of China of 2013 [grant number 2013226027-07] and by the Program for Liaoning Innovative Research Team in University [grant number LT2014018].