New compound with DNA Topo I inhibitory activity purified from Penicillium oxalicum HSY05

Abstract

Strain HSY05 was isolated from sea sediment collected from the South China Sea and was later identified as Penicillium oxalicum by 16S rDNA sequence analysis. Various chromatographic processes led to the isolation and purification of two metabolites from the fermentation culture of HSY05, including one new compound, 2,2′,4,4′-tetrahyoxy-8′-methyl-6-methoxy-acyl-ethyl-diphenylmethanone (1), and a known compound secalonic acid D (SAD, 2), as characterised by UV, IR, 1D, 2D-NMR and MS data. The inhibitory activities against topoisomerase I of these two compounds were evaluated. The result showed that in addition to the known topo I inhibitor SAD (2), compound 1 also exhibited a moderate inhibitory effect.

Keywords::

• Penicillium oxalicum
• marine fungal metabolites
• DNA topoisomerase I inhibitory activity

Acknowledgements

We would like to thank Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The paper was supported by National Natural Science Foundation of China [grant number 81202425]; Scientific Research Fund of Liaoning Provincial Education Department [grant number L2011178] and program for Innovative Research Team of the Ministry of Education and program for Liaoning Innovative Research Team in University.