Two new ent-atisanes from the root of Euphorbia fischeriana Steud.

Abstract

Two new ent-atisanes ent-1β,3β,16β,17-tetrahydroxyatisane (1), ent-1β,3α,16β,17-tetrahydroxyatisane (2) together with 11 known diterpenes were isolated from the anti-tumour activity fraction of Euphorbia fischeriana Steud. The compounds were identified by detailed spectroscopic analysis, including extensive 2D-NMR experiments. X-ray analysis was applied to determine the structure of compound 2. All 13 compounds were screened for cytotoxicity in vitro against human tumour MCF-7, HepG-2 and SGC-7901 cell lines. Compounds 1 and 2 showed the inhibitory effects against MCF-7 with IC₅₀ levels of 23.21 and 15.42 μM; simultaneously, compounds 4, 6, 8 and 11 also had definite inhibitory effect against different cell lines.

Keywords:

• ent-atisane
• Euphorbia fischeriana Steud
• X-ray
• stereoismers
• anti-tumour activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Notes

^1. These authors contributed equally to this study.

Additional information

Funding

This work was supported by the National Basic Research Program of China [973 Program grant number 2013CB531801] and the Foundation of Research and Innovation for Graduate Student of Heilongjiang Province [grant number YJSCX2012-354HLJ].