Abstract
Fractionation of the methanol extract of the leaves of Oricia renieri and Oricia suaveolens (Rutaceae) led to the isolation of 13 compounds including the hitherto unknown furoquinoline alkaloid named 6,7-methylenedioxy-5-hydroxy-8-methoxy-dictamnine (1) and a flavanone glycoside named 5-hydroxy-4′-methoxy-7-O-[α-l-rhamnopyranosyl(1‴→5″)-β-d-apiofuranosyl]-flavanoside (2), together with 11 known compounds (3–13). The structures of the compounds were determined by comprehensive analyses of their 1D and 2D NMR, mass spectral data and comparison. All compounds isolated were examined for their activity against human carcinoma cell lines. The alkaloids 1, 5, 12, 13 and the phenolic 2, 8, 11 tested compounds exhibited non-selective moderate cytotoxic activity with IC50 8.7–15.9 μM whereas compounds 3, 4, 6, 7, 9 and 10 showed low activity.
Acknowledgements
We are also grateful to the Organic Chemistry Department of the University of Reading, Reading, UK and University of Keele, Keele, UK, for recording the NMR measurements and Department of Chemistry, Bielefeld University, Germany for recording Mass spectra and the General Hospital Douala for evaluating the cytotoxicity activity.
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplementary material
Experimental details relating to this article are available online at http://dx.doi.org/10.1080/14786419.2015.1057727 alongside Figures S1–S17 and Tables S1–S3.