Urease inhibitory profile of extracts and chemical constituents of Pistacia atlantica ssp. cabulica Stocks

Abstract

The current study was designed to evaluate the urease inhibitory profile of extract and fractions of Pistacia atlantica ssp. cabulica Stocks followed by bioactivity-guided isolated compounds. The crude extract was found significantly active with urease inhibitor (95.40% at 0.2 mg/mL) with IC₅₀ values of 32.0 ± 0.28 μg/mL. Upon fractionation, ethyl acetate fraction displayed 100% urease inhibition with IC₅₀ values of 19.9 ± 0.51 μg/mL at 0.2 mg/mL. However, n-hexane and chloroform fractions exhibited insignificant urease inhibition. Similarly, the isolated compound, transilitin (1) and dihydro luteolin (2) demonstrated marked urease attenuation with 95 and 98% respectively, at 0.15 mg/mL. Both the isolated compounds showed marked potency with IC₅₀ values of 8.54 ± 0.54 and 9.58 ± 2.22 μg/mL, respectively. In short, both the extract and fractions and isolated compounds showed marked urease inhibition and thus a useful natural source of urease inhibition.

Graphical abstract showing the image Pistacia atlantica, chemical structure of isolated compounds and urease enzyme.

Keywords:

• Pistacia atlantica ssp. cabulica Stocks
• chemical constituents
• urease inhibitory activity

Acknowledgement

The authors are extremely thankful to Higher Education Commission (HEC) Pakistan for providing financial support.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by Higher Education Commission (HEC) Pakistan.