A new macrolactin antibiotic from deep sea-derived bacteria Bacillus subtilis B5

Abstract

A new macrolactin derivate, 7-O-2′E-butenoyl macrolactin A (1), together with three known macrolactin compounds, macrolactin A (2), 7-O-malonyl macrolactin A (3) and 7-O-succinyl macrolactin A (4), was isolated from the bacterial strain Bacillus subtilis B5, which was isolated from the 3000 m deep sea sediment of the Southwest Pacific Ocean. The structures of the new compounds were assigned by spectroscopic methods including 1-D/2-D NMR and MS analysis techniques. Compounds 1 and 2 demonstrated antifungal activities against tea pathogenic fungi Pestalotiopsis theae and Colletotrichum gloeosporioides.

Keywords:

• Bacillus subtilis B5
• 7-O-2′E-butenoyl macrolactin A
• antifungal activity

Funding

The project was supported by the National Natural Science Foundation of China [grant number 81302652]; the Key Science and Technology Project of Fujian Province, China [grant number 2014Y0068], [grant number 2013N0019]; the Science and Technology Program of Xiamen, Fujian Province, China [grant number 3502Z20143001], [grant number 3502Z20142009]; the South Center for Marine Research [grant number 14GYY023NF23], [grant number 13GZP002NF08]; China Ocean Mineral Resources R&D Association [grant number COMRA DY125-15-T-07].