Phytoecdysteroids-containing extract from Stachys hissarica plant and its wound-healing activity

Abstract

A number of phytoecdysteroid compounds, such as ecdysterone, polipodin V, 2-deoxy-20-hydroxyecdysone, integristeron A and 2-deoxydizon were isolated from Stachys hissarica plant and their structures were confirmed by NMR, mass and IR spectroscopy. In addition, the biological activity of the S. hissarica plant’s extract was tested on rats for wound healing activity. It was shown that the extract at repeated oral (per os) administration at a dose of 10 mg/kg speeds up the healing process of linear skin wounds in rats. The wound-healing activity of S. hissarica extract is confirmed to be effective and exceeds known drug methyluracil (2,4-dioxo-6-methyl-1,2,3,4-tetrahydropyrimidine), especially in case of alloxan induced diabetic animals.

Keywords:

• Phytoecdysteroids
• Stachys hissarica
• ecdysteron
• wound-healing activity
• extract

Acknowledgements

Authors would like to thank the ICPS (Uzbek Academy of Sciences) for providing all necessary equipment, chemicals and animals for the study.