A new cerebroside and the cytotoxic constituents isolated from Xylaria allantoidea SWUF76

Abstract

A new cerebroside, namely allantoside (1), and 10 known compounds (2–11) were isolated from Xylaria allantoidea SWUF76. The structure of compound 1 was determined by comprehensive spectroscopic analysis including 1D and 2D nuclear magnetic resonance (NMR) as well as high-resolution electron ionisation mass spectrometry (HREIMS) and electrospray ionisation mass spectrometry (ESIMS). Compounds 1, 4, 5, 6, 7, 8 and 11 were evaluated for cytotoxic activities against cancer cell lines (Hela, HT29, HCT116 and MCF-7) and normal Vero cell lines by MTT assay. Compounds 6 and 7 exhibited anticancer activity after 24 h of treatment. Compound 7 showed significant cytotoxicity against Hela (IC₅₀ = 2.24 μg/mL), HT29 (IC₅₀ = 2.51 μg/mL), HCT116 (IC₅₀ = 3.50 μg/mL) and MCF-7 (IC₅₀ = 3.77 μg/mL) and Vero (IC₅₀:3.65 μg/mL) cells. Compound 6 showed slight cytotoxicity against all tested cancer cell lines.

Keywords:

• Xylariaceae
• Xylaria allantoidea
• secondary metabolite
• cytotoxicity

Acknowledgements

We would like to thank the Laboratory of Natural Products Research, Faculty of Science and Technology, Phuket Rajabhat Unviversity, Phuket, Thailand for the NMR measurements.