The Proteins from Sika deer antler as potential modulators on cisplatin-induced cytotoxicity in human embryonic kidney 293 cells

Abstract

Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its’ possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD₅₀ value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells. In addition, SDAPR treatment markedly inhibited the levels of TNF-α and IL-6. In conclusion, Sika deer antler protein, a potential modulator, could alleviate cisplatin-induced cytotoxicity in HEK 293 cells.

Keywords:

• Sika deer antler protein
• cytotoxicity
• cisplatin
• potential modulators
• HEK 293 cell

Abbreviations:

• Human embryonic kidney 293 (HEK 293)
• Sika deer antler protein (SDAPR)
• 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
• Dimethyl sulfoxide (DMSO)
• Super oxide dismutase (SOD)
• Glutathione (GSH)
• Lactate dehydrogenase (LDH)
• Malondialdehyde (MDA)
• Bicinchoninic acid (BCA)
• Radio-Immunoprecitation (RIPA)
• Phenymethanesulfonyl fluoride (PMSF)
• Tumour necrosis factor alpha (TNF-α)
• Interleukin-6 (IL-6)
• Sodium dodecyl sulfate (SDS)
• The terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL)