Chemical Composition of Buddleja polystachya Aerial Parts and its Bioactivity against Aedes aegypti

Abstract

A new acylatediridoid glycoside, 6-O-α-L-(2″-acetyl-4″-O-trans-isoferuloyl) rhamnopyranosyl catalpol (9) together with 18 known compounds belonging to the iridoids, flavonoids, triterpene saponin glycosides and phenylethanoids (1–8, 10–18) were isolated from the aerial parts and the flowers of Buddleja polystachya. Their structures were elucidated on the basis of spectroscopic evidence and comparison with that reported in the literature. Promising adulticidal activity was shown for all extracts when tested for adulticidal and larvicidal activities against Ae. aegypti mosquitoes. Therefore, isolated compounds (1–10, 12–14 and 19) were bioassayed for their adulticidal activity. Compound 1 (phytol) was highly active with an LD₅₀ value of 1.27 ± 0.08 μg/mosquito against adult female Ae. aegypti.

Keywords:

• Buddleja polystachya
• adulticidal
• larvicidal
• acylated iridoid glycosides

Acknowledgement

We thank Dr. Alden S. Estep and Miss Jessica Louton and (USDA-ARS, CMAVE, Gainesville, FL) for mosquito bioassays.