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Natural Product Research
Formerly Natural Product Letters
Volume 32, 2018 - Issue 24
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Short Communication

A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

Emmanuel V. PontualDepartamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, BrazilCorrespondence[email protected], [email protected]
,
Divar F. Pires-NetoDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brazil
,
Karina FraigeDepartamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Brazil
,
Taciana M. M. HiginoDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brazil
,
Belany E. A. CarvalhoDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil
,
Neyla M. P. AlvesDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brazil
,
Thâmarah A. LimaDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil
,
Russolina B. ZingaliInstituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
,
Luana C. B. B. CoelhoDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil
,
Vanderlan S. BolzaniDepartamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Brazil
,
Regina C. B. Q. FigueiredoDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brazil
,
Thiago H. NapoleãoDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil
&
Patrícia M. G. PaivaDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil
 show all
Pages 2940-2944 | Received 05 Jul 2017, Accepted 26 Sep 2017, Published online: 19 Oct 2017
 
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Abstract

In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 ± 6.62 and 41.20 ± 4.28 μg/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract.

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