Abstract
The aerial parts of Baccharis dracunculifolia (BdE) is used in the Brazilian folk medicine to treat inflammatory conditions. Here we examined the ability of free and liposomal BdE to modulate reactive oxygen species generation in human neutrophils in vitro and zymosan-induced acute joint inflammation in Wistar rats. We prepared biocompatible liposomes of soya phosphatidylcholine and cholesterol with low diameter, homogeneous size distribution, and neutral surface charge. Free BdE decreased joint swelling, total leucocyte and neutrophil infiltration, and the synovial levels of tumour necrosis factor-α and interleukins 6 and 1β. Incorporation of BdE into liposomes preserved its capacity to inhibit the neutrophil superoxide anion and total reactive oxygen species generation, and improved its anti-inflammatory effect in vivo by decreasing the effective BdE dose by nearly sixfold. The same liposome type lowered the effective dose of caffeic acid by nearly sixteenfold. Therefore, incorporation of BdE into phosphatidylcholine-cholesterol liposomes improves its anti-inflammatory effect.
![](/cms/asset/b0f33b54-3875-476e-970a-bab25cbf7536/gnpl_a_1448809_uf0001_oc.jpg)
Acknowledgements
We thank Dr Larissa F. Marchi and Prof. Marcelo Dias-Baruffi, PhD, for scientific discussions, and Mrs Nadir Mazzucato and Mr Alcides S. Pereira for their technical assistance at the School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.