Cure of human diabetic neuropathy by HPLC validated bark extract of Onosma echioides L. root

Abstract

HPLC validated hexane bark extract of Onosma echioides L. root (OE) was evaluated for cure of human diabetic neuropathy in human neuroblastoma cell line. HPLC analysis was performed. Human neuroblastoma cells were grouped into control, normal glucose, high glucose (HG) and HG plus different concentrations of OE extract (10, 25 and 50 μg/mL). MTT, DCFH-DA staining and nuclear condensation assays were performed on neuroblastoma cells to evaluate antiproliferative activity, ROS activity level and apoptotic effect of OE. HPLC analysis revealed the existence of maximum yield of shikonin in n-hexane extract of OE. Exposure with different concentrations of OE effectively decreased ROS level and apoptosis of cells and as a result improved the viability of cells in a dose dependent manner in response to HG-induced oxidative stress. Thus, OE possesses the property to cure human diabetic neuropathy and further can be clinically tested for its use in diabetic neuropathy.

Keywords:

• Onosma echioides
• high performance liquid chromatography
• ROS
• apoptosis
• neuroblastoma cell
• neuropathy

Acknowledgments

First author acknowledges to University Grant Commission (UGC), Government of India for providing Senior Research Fellowship (201213-MANF-2012-13-MUS-UTT-14306) to this research work. All the authors are thankful to Integral University, Lucknow and University of Lucknow for providing all the facilities to carry out this research work (Manuscript Communication Number: IU/R&D/2017-MCN00064).