Isolation and structure determination of a new indene derivative from endophytic fungus Aspergillus flavipes Y-62

Abstract

As part of the search for naturally derived secondary metabolites, a novel indene derivative, compound 1, together with nine known metabolites (2–10) have been purified from an ethyl acetate extract of the plant-associated fungus Aspergillus flavipes Y-62, isolated from Suaeda glauca (Bunge) Bunge which was collected along Zhoushan coast, Zhejiang province, East China. The structure of the new compound 1 was elucidated by extensive use of spectroscopic techniques like 1D, 2D NMR, and HR-TOF-MS, while the known metabolites were established based on both spectral methods as well as by comparison with the previous literature. Compound 1 exhibited antimicrobial activities against the gram-negative pathogen Pseudomonas aeruginosa and Klebsiella pneumoniae with equal MIC values of 32 µg/ml.

Keywords:

• Endophytic fungi
• Indene derivative
• secondary metabolites
• Antimicrobial activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work is financially supported by the National Key Research and Development Program of China (No. 2018YFC0311002) and NSFC (Nos. 81573306 and 41876148).