Two highly oxygenated Ergosterols from cultures of the Basidiomycete Conocybe siliginea

Abstract

Two highly oxygenated ergosterols, (22E,24R)-3β,5α,6β,9α,14α,25-hexhydroxyergosta-7,22-diene (1), and (22E,24R)-3β,5α,6β,14α,25-pentahydroxyergosta-7,9(11),22-triene (2), together with two known ones, have been isolated from cultures of the basidiomycete Conocybe siliginea. Their structures were elucidated on the basis of extensive spectroscopic means. Two new compounds (1 and 2) were tested for their nitric oxide synthase inhibitory activities. However, none of them showed any activity (IC₅₀ > 40 μM).

Keywords:

• Conocybe siliginea
• ergosterol
• isolation and structural elucidation

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by the National Key Research and Development Program of China (2017YFC1704007), National Natural Science Foundation of China (81773590, 81561148013), Thailand Research Fund (grant No. DBG5980002), the Key Projects of Technological Innovation of Hubei Province (No. 2016ACA138), and the Fundamental Research Funds for the Central University, South-Central University for Nationalities (CZP18005, CZQ17010, CZQ17008, CZT18014, CZT18013). The authors thank the Analytical & Measuring Center, School of Pharmaceutical Sciences, South-Central University for Nationalities for the spectra test.