Cuminum cyminum fruits as source of luteolin- 7-O-glucoside, potent cytotoxic flavonoid against breast cancer cell lines

Abstract

Cuminum cyminum is famous for its spicy fruits used for culinary and therapeutic properties worldwide. Brine shrimp test was performed for detecting cytotoxic fractions and subfractions. Ethyl acetate (EA) and hexane (HE) fractions demonstrated LC₅₀ of 52.40 and 60.77 µg/ml against Artemia salina while other fractions showed no toxicity (LC₅₀> 500 µg/ml). Bioguided elucidation of EA and HE fractions were carried out and cytotoxicity of pure compounds were investigated against breast cancer cell lines (MCF-7 and MDA-MB-231) and normal cell line (NIH/3T3) by MTT assay. Four flavone structures as luteolin, apigenin, luteolin-7-O-glucoside and apigenin-7-O-glucoside from EA and cuminoid A from HE were purified and identified. Luteolin-7-O-glucoside demonstrated potent anticancer activities against MCF-7 cell line (IC₅₀ of 3.98 µg/ml) with selectivity index of 8.0. In conclusion, flavonoids especially luteolin-7-O-glucoside play a significant role in cytotoxic effect of C. cyminum fruits and can be introduced as candidate for chemopreventive and chemotherapeutic drugs.

Graphical Abstract

Keywords:

• Cumin
• seed
• cytotoxic
• Luteolin
• Apigenin
• Cuminoid A

Disclosure statement

The authors declare that there is no conflict of interest.

Additional information

Funding

This research was supported by a grant of Tehran University of Medical Sciences and Health Services (No. 30990).