Abstract
Development of a novel agent for control of schistosomiasis is a mandate. In-vitro anti-schistosomal activity of the aerial parts of Huernia saudi-arabica were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-β-p-hydroxy-benzoyl-20-O-acetyl-boucerin-3-O-β-D-glucopyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-O-β-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized via spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). In-vitro bioassays on the viability (mobility, morphological changes and mortality) of Schistosoma mansoni adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 μg/ml of EE and 2.6, 5.2, 13, 26 and 52 μM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced in-vitro anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate in-vitro cytotoxicity with IC50 of 8.48 µg/ml.
Graphical Abstract
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Disclosure statement
The authors declare that they have no competing interests.
Acknowledgements
Authors would like to thank Dr. Nahid Wally, College of Science, King Abdelaziz University, Jeddah, Saudi Arabia who kindly identified Huernia saudi-arabica plant.