Coprinus comatus filtrate extract, a novel neuroprotective agent of natural origin

Abstract

In vitro acetylholinesterase (AChE) inhibitory activity of an autochthonous sample of the mushroom Coprinus comatus (encompassing fruiting body FB, mycelia M and filtrate F from the submerged cultivation) was the subject of this study. C. comatus F extract exhibited rather potent anti-AChE activity (73.0 ± 1.5%) at in liquid conditions, comparable to those of the conventional drug donepezil (80.6 ± 1.4%). Also, the same extract exhibited high anti-AChE activity (1 µg) in solid. While its FTIR spectrum indicated the presence of phenolic compounds, quercetin (28.1 µg g⁻¹ d.w.) was found to affect the observed bioactivity (59.8 ± 0.9%). This is the first report of profound anti-AChE activity of any C. comatus extract, a medicinal mushroom that has been successfully cultivated in P.R. China, due to the demanding needs of food industry.

Graphical Abstract

Keywords:

• Coprinus comatus filtrate extract
• AChE inhibitory activity
• polyphenolic profile
• quercetin
• new AD health promoting product

Acknowledgments

Author (Boris Pejin) dedicates this study to the memory of beloved Diana, the late Princess of Wales.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was kindly supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia [Research grants Nos. 172053, 172058, 173040, 43002, 43010 and 46001].