Antitumor activity on human bladder cancer T-24 cells and composition analysis of the core of Camellia osmantha fruit

Abstract

In this study, antitumor activity and composition analysis of the core of Camellia osmantha fruit were investigated. The cores were extracted by 80% ethanol to obtain the crude extract (TF), which was successively distributed by different concentrations of ethanol with MCI resin to obtain three different parts (Frs 1 to 3). Cytotoxicity activities showed that TF, Fr-2, and Fr-3 exhibited good inhibition against T-24 with IC₅₀ values of 6.7 ± 1.3, 6.9 ± 0.9, and 6.7 ± 1.7 μg/mL, respectively. Fr-3 has a strong inhibitory activity on T-24 cells, mainly due to effectively increasing the release of intracellular calcium ions and reactive oxygen species. In addition, Fr-3 can inhibit T-24 cells migration and invasion. Further composition analysis on Fr-3 was detected by LC-Q-TOFMS implying the main components to be ellagic acid and its derivatives.

Graphical Abstract

Keywords:

• Camellia osmantha
• composition analysis
• T-24
• LC-Q-TOFMS
• cell migration

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by 2017 Science and Technology Major Project of Guangxi (grant number AA17204058-21) and the Natural Science Foundation of Guangxi Province (grant number 2017GXNSFAA198034).