A new 5α,8α-epidioxysterol with immunosuppressive activity from the South China Sea soft coral Sinularia sp.

Abstract

A new 5α,8α-epidioxysterol, namely yalongsterol A (1), along with two known related steroids 5α,8α-epidioxy-24-methyl-cholesta-6,24(28)-dien-3β-ol (2) and (22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,22-dien-3β-ol (3), were isolated from the South China Sea soft coral Sinularia sp. Their structures were established by extensive spectroscopic analyses and comparisons of the spectral data with those reported in the literature. In bioassay, compounds 1–3 showed moderate immunosuppressive activities against T and/or B lymphocyte cells with IC₅₀ values ranging from 19.30 to 59.49 μM, and low cytotoxicity on murine splenocytes with CC₅₀ values ranging from 40.88 to 62.29 μM.

keywords:

• Immunosuppressive activity
• Soft coral
• Sinularia sp.
• 5α,8α-Epidioxysterol
• Yalongsterol A

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research work was financially supported by the National Key Research and Development Program of China (No. 2018YFC0310903), National Natural Science Foundation of China (Nos. 81520108028, 21672230, 41876194, 41506187, 81603022, 81811530284), the National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers (No. U1606403), and the State Key Laboratory of Drug Research/Shanghai Institute of Materia Medica Project (SIMM 1705ZZ-01).