Carbamothioic S-acid derivative and kigamicins, the activated production of silent metabolites in Amycolatopsis alba DSM 44262Δabm9 elicited by N-acetyl-D-glucosamine

Abstract

One new carbamothioic S-acid derivative (1) and five known kigamicin derivatives (2–6) were isolated from the fermentation extract of Amycolatopsis alba DSM 44262Δabm9 elicited by N-acetyl-D-glucosamine. HPLC-DAD-UV analyses indicated that the DSM 44262Δabm9 strain did not produce these metabolites originally and the production of 1–6 was induced by adding 25 mM N-acetyl-D-glucosamine in the culture medium. The structures of 1–6 were identified on the basis of NMR spectroscopic data and high-resolution ESIMS. These results highlight that addition of N-acetyl-D-glucosamine in the microbial culture medium could activate cryptic gene expression, induce and increase the production of new or known secondary metabolites.

Graphical Abstract

Keywords:

• Amycolatopsis alba DSM 44262Δabm9
• carbamothioic S-acid
• kigamicins
• N-acetyl-D-glucosamine
• elicitation

Additional information

Funding

This research was supported by the National Natural Science Foundation of China (81530091, 81673317), Science Foundation of Two sides of Strait (U1405223) and Program for Changjiang Scholars and Innovative Research Team in University (IRT_17R68).