Sesquiterpenes produced by Pestalotiopsis microspora HF 12440 isolated from Artocarpus heterophyllus

Abstract

A drimane-type sesquiterpene, (+)-dendocarbin L (1) together with two bisabolane-type sesquiterpenes, (+)-sydonic acid (2) and (+)-sydowic acid (3) were isolated from the mycelium of Pestalotiopsis microspora HF 12440, an endophytic fungus from the stem of Artocarpus heterophyllus. The structures of all compounds were elucidated using spectroscopic methods and by comparison with the literature. Compound 1 was isolated from the fungi for the first time, compounds 2 and 3 were firstly obtained from this endophytic fungus. Compound 3 showed cytotoxicity (IC₅₀ 2.56 μg/mL) against murine leukemia P-388 cells.

Graphical Abstract

Keywords:

• Artocarpus heterophyllus
• cytotoxicity
• Pestalotiopsis microspora
• sesquiterpene

Acknowledgements

PMDSU Scholarship (Ministry of Research, Technology and Higher Education of Indonesia) under Grant 1511/E4.4/2015 for financial support; Indonesian Culture Collection (InaCC), Research Center for Biology – Indonesian Institute of Sciences (LIPI) for the identification of endophytic fungus; Prof. Yana Maolana Syah and Dr. Elvira Hermawati (Bandung Institute of Technology) for obtaining NMR and MS spectra.

Disclosure statement

No potential conflict of interest was reported by the authors.