Cytotoxic triterpenoid saponins from the root of Anemone tomentosa (Maxim.) Pei

Abstract

Two new triterpenoid saponins, tomentoside T₁ (1) and tomentoside T₂ (2), together with eleven known compounds (3–13) were obtained from ethyl acetate extracts of Anemone tomentosa (Maxim.) Pei by bioactivity-guided isolation. Their structures were elucidated by chemical and spectral analysis, including 1D, 2D NMR data and HRESIMS. The cytotoxic activity of all isolated saponins was evaluated on MDA-MB-231 and PC-3 cell lines. Compounds 5 and 7 showed significant cytotoxicity against with MDA-MB-231 cell and PC-3 cell with IC₅₀ values of 3.73–4.21 μM and 3.53–5.37 μM, respectively.

Graphical Abstract

Keywords:

• Anemone tomentosa (Maxim.) Pei
• triterpenoid saponins
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by National Natural Science Foundation of China [grant number 81373900]; the fourth national (Liaoning Provinces) survey on Chinses materia medica resources program [grant number 2018018].