Chemical and activity investigation on metabolites produced by an endophytic fungi Psathyrella candolleana from the seed of Ginkgo biloba

Abstract

An endophytic fungus that can produce flavonoids was isolated from the seed of Ginkgo biloba cultured in Czapek-Dox medium and chromatographic separation of the ethyl acetate extract of the broth and mycelium led to the isolation of ergosterol (1), 2′-hydroxychalcone (2), myristic acid (3), cis-9-octadecenoamide (4), quercetin (5), carboxybenzene (6), uracil (7) and nicotinamide (8). This study is the first to report of the isolation of the endophytic fungus Psathyrella candolleana from the seed of Ginkgo biloba with complete assignments of 1–8. Compound (5) exhibited strong antioxidant activity of diphenyl picryl hydrazinyl (CL₅₀ 14.538 μg/mg) and compounds (5), (6) and (8) have antibacterial activity against Staphylococcus aureus (MIC 0.3906, 0.7812 and 6.25 mg/mL).

Graphical Abstract

Keywords:

• Ginkgo biloba
• endophytic fungi
• Psathyrella candolleana

Disclosure statement

No potential conflict of interest was reported by the authors.

Figure 1. Compounds 1–8 structure.

Additional information

Funding

This work was supported by a key project funded by science and technology department of Zhejiang province (No. 2015A22163).