New metabolites produced by endophyte Clonostachys rosea B5 − 2

Abstract

The endophytic fungus, Clonostachys rosea B5-2 was isolated from mangrove plants and subjected to the one strain many compounds (OSMAC) methodology. By this approach, it was found that modification of the culture media enhanced the production of secondary metabolites by C. rosea B5-2. The apple juice supplemented solid rice media led to significant changes in the secondary metabolism of the fungus, C. rosea B5-2, and induced the production of four new compounds, (-)-dihydrovertinolide (2), and clonostach acids A (3), B (4), and C (5) together with the known compound, (-)-vertinolide (1). The new compound (-)-dihydrovertinolide (2) exhibited phytotoxicity against lettuce seedlings at a concentration of 50 mg L⁻¹.

Keywords:

• Endophyte
• Clonostachys rosea
• OSMAC
• (-)-Vertinolide
• phytotoxicity

Acknowledgments

This investigation was financially supported by Directorate General of Scientific Resources, Technology and Higher Education, Ministry of Research, Technology and Higher Education, Indonesia (World Class Professor Grant, Number T/83/D2.3/KK.04.05/2019 for US). The authors thank Dr. Takako Aboshi in Yamagata University for MS experimental supports.