Two new diterpenoids with their antiproliferative activities from the supercritical fluid extraction of Croton crassifolius root

Abstract

Supercritical fluid extraction was applied to obtain the lower polarity extracts from Croton crassifolius roots, and chemical investigation of which led to the isolation and identification of two new diterpenoids, named crassifolius P (1) and crassifolius Q (2). In vitro anti-proliferative activities of compounds 1 and 2 on A549, Hep-G2 and Hela tumor cell lines were evaluated. The two new compounds exhibited obvious selectivity to tumor cells with IC₅₀ values ranging from 20.43 ± 1.18 μM to 25.72 ± 1.32 μM.

Graphical Abstract

Keywords:

• Croton crassifolius
• diterpenoid
• antiproliferative

Acknowledgments

The authors would like to thank Prof. Chenggang Shan for identifying the species of C. crassifoliu.

Disclosure statement

The authors declare that they have no conflicts of interest.

Additional information

Funding

This work was supported by the [Major Scientific and Technological Innovation Project of Shandong, China] under Grant [2019JZZY020612]; [Provincial Key Research and Development Program of Shandong] under Grant [2019LYXZ011, 2019LYXZ025]; [Shandong Province: ”Double-Hundred Talent Plan” on 100 Foreign Experts and 100 Foreign Expert Teams] under Grant [WST2017004]; [Provincial Natural Science Foundation of Shandong] under Grant [ZR2019MC072] and [Taishan Scholars’s Program of Shandong-Jin-yue Sun].