Two new norlignans from the aerial parts of Pouzolzia sanguinea (Blume) Merr

Abstract

Two new norlignans, pouzolignan N (1) and pouzolignan O (2), together with five known norlignans, pouzolignan F (3), pouzolignan G (4), pouzolignan H (5), pouzolignan L (6), and gnetifolin F (7) were isolated from the aerial parts of Pouzolzia sanguinea (Blume) Merr. Their chemical structures were elucidated via HR-ESI-MS and NMR spectroscopic methods. Absolute configurations at stereocenters were confirmed by comparisons of CD spectra with those of TD-DFT calculations. Compounds 1–6 exhibited chemical structures unique to Pouzolzia species. At a concentration of 30 µM, compounds 1–7 exhibited weak cytotoxic activity toward CAL27 and MDA-MB-231 cell lines (cell viability from 65.3 ± 0.86 to 98.8 ± 1.23%). They also inhibited anoctamin-1 activity with inhibitory rates from 8.1 ± 0.87 to 24.3 ± 1.41%.

Graphical Abstract

Keywords:

• Pouzolzia sanguinea (Blume) Merr
• Pouzolignan N
• Pouzolignan O
• Nuclear magnetic resonance
• Cytotoxicity
• Anoctamin-1 inhibitor

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 104.01-2016.30.