Secondary metabolites from Garcinia daedalanthera Pierre leaves (Clusiaceae)

Abstract

Two new glycerol esters, (S)-2-hydroxy-3-(octanoyloxy)propyl tetracosanoate (1) and (S)-3-(((S)-11-acetoxy octadecanoyl)oxy)propane-1,2-diyl diacetate (2), and eight known compounds, docosanedioic acid (3), 2,5-dimethylnonadecane (4), lupeol (5), stigmasterol (6), β-sitosterol (7), heptadecanoic acid (8), hexanedioic acid, 1,6-bis[(2R)-ethylhexyl] ester (9), and 1,3-di-O-[2’,2’-di-(p-phenylene)] (10) were isolated from the leaves of Garcinia daedalanthera Pierre, collected from Indonesia. Structural analysis of the isolates was performed using 1 D- and 2 D-NMR, LC- and GC-MS, IR, polarimetry, and UV-visible spectroscopic methods. Cytotoxicity assessments, as well as reactive oxygen species (ROS) analysis of the isolates, were also completed. Lupeol was the only compound found active with an IC₅₀ value of 19.2 µM against HT-29 colon cancer cells. Significant ROS inhibition and induction activity was observed for compounds 4 and 8, respectively.

Keywords:

• Garcinia daedalanthera
• spectroscopic analysis
• fatty acid
• glycerol ester
• reactive oxygen species
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was supported by the College of Pharmacy, Ohio State University, and the Endowment Funds for Education (LPDP), Jakarta, Indonesia. The authors would like to acknowledge Dr. Craig McElroy, College of Pharmacy, Ohio State University, for support in the use of spectroscopic instruments.