Bioactive hydroxypropionylated glucose derivatives from Astragalus bhotanensis

Abstract

Four previously undescribed hydroxypropionylated d-glucose derivatives, astrabhotins A–D (1–4), along with ten known compounds α-d-glucose (5), β-d-glucose (6), quebrachitol (7), 3-hydroxypropionic acid (8), oleic acid (9), isoliquiritigenin (10), liquiritigenin (11), odoratin (12), 7β-hydroxysitosterol (13) and daucosterol (14), were isolated from the roots of Astragalus bhotanensis. Their structures were elucidated based on the analyses of extensive spectroscopic data and physicochemical properties. Astrabhotin A (1) reduced the writhing response remarkably with 52.5% inhibition by acetic acid induced writhing test. The analgesic effect of 1 was stronger than the standard drug aspirin. In addition, compounds 1 and 3 showed significant antioxidant activities with IC₅₀ values of 9.9 ± 0.2 and 7.9 ± 0.4 μg/mL, and exhibited weak or moderate cytotoxicity against HepG2 cells with IC₅₀ values of 106.6 ± 2.7 and 42.0 ± 0.9 μg/mL, respectively.

Keywords:

• Astragalus bhotanensis
• Leguminosae
• hydroxypropionylated glucose derivatives
• analgesic
• cytotoxicity
• antioxidant

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The work was financially supported by the National Natural Science Foundation of China [grant numbers 31170313 and 81641187], and the Innovation Team Project of Dali University for Development and Utilization of Characteristic Medicinal Plants in Western Yunnan & Bai Nationality Medicines [grant number ZKLX2019106].