Chemophenetic study of Ocotea canaliculata (Lauraceae) by UHPLC–HRMS and GNPS

Abstract

The metabolic fingerprint of a non-volatile fraction of Ocotea canaliculata (Rich.) Mez (Lauraceae) leaves was determined by UHPLC–HRMS analysis. Twenty-four compounds were suggestively identified by GNPS-FBMN. The results revealed a large production of flavonoids, mainly flavones and flavanones, a chemical class poorly described in the Ocotea genus. Within the identified compounds, four are being described for the first time in this genus. The major metabolite detected was astilbin, with a concentration corresponding to 23.2 ± 1.58% of the extracts. The expressive content of astilbin also highlights it as a chemical marker for the species. As a species that is classified as a complex, qualitative and semi-quantitative features obtained through the O. canaliculata flavonoid fingerprint can be further used for a more precise circumscription and species-specific characterization.

Graphical Abstract

Keywords:

• Lauraceae
• untargeted metabolomic
• chemophenetic
• flavonoids
• GNPS
• astilbin

Acknowledgements

This is the 804 study of the Technical Series of the Biological Dynamics Project of Forest Fragments (INPA / STRI). The authors acknowledge the assistance of Dr. Alberto Vicentini (INPA), José Luis Camargo, coordinator of the Biological Dynamics of Forest Fragments Project (PDBFF); and PDBFF researcher Dr. Ana Cristina Segalin de Andrade. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors would like to acknowledge the financial support of the National Council for Scientific and Technological Development (CNPq).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors would like to acknowledge the support of the Coordination for the Improvement of Higher Education Personnel (CAPES) and the National Council for Scientific and Technological Development (CNPq). This is the 804 study of the Technical Series of the Biological Dynamics of Forest Fragments Project (INPA/STRI).