Phytochemical profiling of aqeous methanolic leaf extract of Triclisia gilletii by gas chromatography (GC/MS) and liquid chromatography (HPLC-PDA–ESI/MSⁿ) tandem mass spectroscopy (MS): a pointer to its nephroprotection

Abstract

The phytochemical constituents in the aqueous methanolic leaf extract of Triclisia gilletii responsible for its nephroprotective potentials against ethane-1,2-diol induced nephrolithiasis as previously investigated in our laboratory were elucidated. The extract was prepared using 80% aqueous methanol in 72 h, Phytochemical contents of aqueous methanolic extract of Triclisia gilletii (TGME) was identified using both a Thermo Scientific DSQII single quadrupole gas chromatography (GC) and a Thermo Scientific liquid chromatography (LCQ Fleet system) tandem mass spectroscopy. The chromatogram acquisition, detection of mass spectral peaks and their waveform processing were performed using Xcalibur MS Software (Thermo Scientific Inc.). GC-MS analysis revealed the presence of phenols, fatty acids, vitamins and steroids. Likewise, for LC-MS analysis kaempferol and dihydrovomifoliol-O-glucoside were detected. The identified constituents have possible contributively effect on the acclaimed pharmacological potential of Triclisia gilletii against ethane-1,2-diol induced nephrolithiasis.

Graphical Abstract

Keywords:

• Triclisia gilletii
• gas chromatography-mass spectrometry
• liquid chromatography-mass spectrometry
• phytochemicals
• nephroprotection

Acknowledgements

The authors would like to thank the centre for international cooperation and development (CICOPS) university of Pavia, Italy for providing mobility for the research in the award of scholarship to Dr. Olayeriju O.S. in 2018. He also wants to sincerely thank his wife, Mrs. Olayeriju Blessing Olajumoke for allowing smooth review/correction process during their honeymoon.

Disclosure statement

The authors have no conflict of interest to declare.