In vivo study of anti-inflammatory and antinociceptive activities of Copaifera pubiflora Benth oleoresin

Abstract

Copaifera pubiflora Benth oleoresin (CPO) is used as an anti-inflammatory, wound healing, and antimicrobial. This paper reports the cytotoxic, anti-inflammatory, and antinociceptive activities of CPO. CPO (10 mg/kg) did not affect locomotor capacity in the open-field and rotarod tests and was not cytotoxic to CHO-k1, THP-1, and L929 cell lines. It was active in the formalin test at 3 mg/kg by 86 ± 3% and 96 ± 3%, respectively, for the first and second phases. At 10 mg/kg, CPO inhibited 90 ± 7%, the pain in the mechanical hyperalgesia test. In the tail-flick test, CPO at 3 mg/kg affected the tail-flick latencies in mice by 77 ± 20%, which in combination with naloxone was only partially reduced. At 3 mg/kg CPO inhibited 80 ± 12% the carrageenan-induced paw edema, and at 3 mg/kg it reduced by 91 ± 5% the nociception on acetic acid-induced abdominal writhing. Therefore, CPO possesses anti-inflammatory and antinociceptive activities.

Graphical Abstract

Keywords:

• Copaifera oleoresin
• Copaiba oil
• anti-inflammatory
• analgesic
• Copaifera pubiflora

Acknowledgements

We thank São Paulo Research Foundation (FAPESP) grant # 2011/13630-7, CAPES and CNPQ for financial support.

Disclosure statement

No potential conflict of interest was reported by the authors.