Elastase inhibitory activity of secondary metabolites from the fungus Virgaria nigra CF-231658

Abstract

Three known compounds were isolated from Virgaria nigra CF-231658; 2,7-dihydroxy naphthalene (1), virgaricin B (2) and virgaricin (3). The isolated compounds was obtained from liquid-state and agar-supported fermentation using Amberlite XAD-16 solid-phase extraction during the cultivation step. Their structures were elucidated on the basis of 1D and 2D NMR as well as HRMS spectroscopic analyses. The isolated compounds were examined for their ability to inhibit elastase using normal human diploid fibroblasts. Compound 2 displayed the most potent activity with 76.7 ± 2.12% inhibition of the enzyme activity at 5 μM concentration.

GRAPHICAL ABSTRACT

Keywords:

• Virgaria nigra CF-231658
• 2,7-dihydroxy naphthalene
• virgaricin B
• virgaricin
• anti-elastase activity

Conflicts of interest

The authors declare no conflict of interest.

Additional information

Funding

This work has been financially supported by EU under the frame of MICROSMETICS project (FP7-PEOPLE- Industry-Academia Partnerships and Pathways), Grant agreement No. 612276.