The role of active metabolites isolated from Jasminum multiflorum flowers against hepatitis C virus infection and related hepatocellular carcinoma

Abstract

Jasminum multiflorum Burm. f. (J. multiflorum) is an ornamental plant with traditional medicinal importance. This study aims to evaluate the activity of J. multiflorum isolated compounds against hepatocellular carcinoma cells infected with hepatitis C virus (HCV) in vitro. The in vitro anti-viral and anti-oncogenic-related activity were validated by anchorage-independent assay plus transwell migration/invasion and spreading assay. In addition to chromatographic isolation of the active metabolites. The flower extract demonstrated a significant antiviral potential through reducing active viral replication by more than 90%. Study results credit this to specific reduction of viral NS5A and cellular EphA2 protein levels. Molecular docking analysis proved the role of the isolated compounds especially multifloroside, jasfloroside A and jasfloroside B as possible anti HCV molecules.

Graphical Abstract

Keywords:

• Hepatitis C virus
• Jasminum multiflorum
• secoiridoids
• NS5A
• EphA2

Acknowledgements

We acknowledge Prof. Michael Wink and Dr. Mansour Ahmed (Heidelberg University, Germany) for giving us the opportunity to collect HPLC-PDA-MS/MS data. And all authors acknowledge the taxonomist Prof. Dr. Abdel Megeed Ali, Agricultural Research Centre, Cairo, Egypt, for the botanical classification of the plant species. The authors would like to thank Dr. Abdulrahman Mohamed Saleh, Al-Azhar University, Cairo, Egypt (e-mail: [email protected]) for providing the docking data.

Disclosure statement

There are no conflicts to declare.