Isolation, characterisation, antifungal activity and validated UPLC/MS/MS method for quantification of novel compound from Artemisia tournefortiana Reichb

Abstract

Investigation into the chemical diversity of Artemisia tournefortiana resulted in isolation of one novel compound named tournefortin A and two known artemetin and tournefortin B bioactive compounds. Tournefortin B is first time obtained from natural source. The structure of all the isolated compounds were elucidated by detailed 1D and 2D NMR including HSQC, HMBC, ¹H-¹HOSY and NOESY spectroscopic techniques. Minimum inhibitory concentration (MIC) of all the tested compounds against tested fungal strains lies between 0.4 and 6.4 µg/mL and lowest MIC of 0.4 µg/mL of compound tournefortin A was found against Alternaria alternate. All the isolated compounds were quantified through UPLC/MS/MS and the developed method will serve as a first fingerprint method for the rapid determination of these phytomolecules in various plant extracts. Tournefortin B was found to be present in higher concentration. The higher antifungal effect of the isolated compounds suggests that this plant could act as potential source of antimicrobial agents.

Keywords:

• Artemisia tournefortiana Reichb
• asteraceae
• tournefortin A
• UPLC/MS/MS
• antifungal activity

Acknowledgements

The authors gratefully acknowledge Director, CSIR-IHBT Palampur (HP), India, for providing the needed facilities during the course of the research. We are greatful to prof. A. R. Naqshi and Dr. Anzar A. Khuroo, centre for plant taxonomy and Biodiversity, University of Kashmir, Hazratbal, Srinagar for botanical identification of plant material. Thanks are due to CSIR, HRDG, New delhi, India for awarding research Associate (RA) to Dr. Mahpara Qadir under file no. 09/251(0164)2020 EMR-1.

Disclosure statement

Authors reported no potential conflict of interest.