Isolation, identification, and quantification of triterpene saponins in the fresh fruits of Panax notoginseng

Abstract

This study is to develop a method for isolation, identification, and quantitative determination of dammarane-type triterpene saponins in the Panax notoginseng fruits (PNF). The saponins were isolated by a serious of chromatographic methods, and their structures were elucidated on the basis of spectroscopic evidence and comparison with those of literature reports. Quantitative assay was performed on an ultra-performance liquid chromatography-UV (UPLC-UV) method. As a result, 22 saponins were isolated from the extract of PNF, among them, compound 1 was a new saponin, named as malonylgypenoside IX, compounds 3–10, and 14–18 were isolated from the PNF for the first time. As to quantitative analysis, the calibration curves showed good linearity (r > 0.998) within the concentration range, and the method validation provided good reproducibility and sensitivity for the quantification of eight major saponins with precision and accuracy of less than 3.0%.

GRAPHICAL ABSTRACT

Keywords:

• Panax notoginseng
• fruits
• saponins
• NMR
• quantification

Supplementary material

Supplementary material relating to this article is available online, alongside Figures S1−S11 and Tables S1−S3.

Additional information

Funding

The authors thank the National Key Research and Development Program of China (2017YFC1702102). The General Research Subject of Higher Education in Jilin Province (JGJX2020D547), and the Project of the Jilin Provincial Department of Science and Technology, China (20170203004YY).