Identification and quantification of cyclitols and sugars isolated from different morphological parts of Raphanus sativus L

Abstract

Through this study, we aimed to develop a new analytical method for identification and quantification of sugars and cyclitols isolated from different morphological parts of Raphanus sativus L (R. sativus). Accelerated solvent extraction with water was involved for targets extraction. Solid phase extraction was used for purification and preconcentration, while high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) was used for chromatographic analyses. A short method of only 30 min for a single analysis was developed finally. The obtained results, allowed for quantification of eight targets, i.e., three cyclitols (D-pinitol, allo-inositol and scyllo-inositol) and five sugars (xylose, D-mannose, D-fructose, D-glucose and sucrose) that were determined simultaneously using a single analysis. The developed method can be applied in industry as a routine method for analysis of sugars and cyclitols from different sources.

Graphical abstract

Keywords:

• Cyclitols and sugars
• HPLC-ELSD
• method development
• Raphanus sativus L.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financed from grant No. BIOSTRATEG2/298205/9/NCBR/2016) by the National Center for Research and Development (Warsaw, Poland). The help provided by our colleague Rafińska Katarzyna is greatly acknowledged.