Antimicrobial and antioxidant activities of secondary metabolites from endophytic fungus Botryosphaeria fabicerciana (MGN23-3) associated to Morus nigra L

Abstract

This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analysed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed towards B. cereus, B. subtilis, and S. aureus, respectively. The cytotoxicity analyses showed CC₅₀ of 115 μg/mL. The crude extract showed antioxidant activity by the DPPH, ABTS, and FRAP assays. Therefore, the extract of the endophytic fungus presented biotechnological potential as an antibacterial and antioxidant agent.

Graphical Abstract

Keywords:

• Endophytic fungi
• mellein
• β-orcinaldehyde
• antibacterial activity
• antioxidant activity
• Morus nigra L

Acknowledgements

The authors thank to the Research Support Center Complex of the University of Maringa – Brazil (COMCAP-UEM).

Disclosure statement

No potential conflicts of interest are reported by the authors.

Additional information

Funding

This work was supported by the CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) (scholarships - Finance code 001); the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (307603/2017-2); and the SETI/UGF (TC n.65/2018). Secretaria de Ciência, Tecnologia e Ensino Superior, Governo do Estado de Parana.