Influence of methyl jasmonate and salicylic acid on Rhamnetin production in adventitious root cultures of Baccharoides anthelmintica (L.) Moench

Abstract

We developed adventitious roots from the leaf explants of Baccharoides anthelmintica (L.) Moench (syn. Vernonia anthelmintica) cultured on Murashige and Skoog (MS) medium. We tested MS and White’s media fortified with different Indole Butyric Acid concentrations for their effect on root biomass production. MS medium supplemented with 1 mg L⁻¹ IBA produced the highest biomass of adventitious roots compared to White’s medium. HPLC and LC-Q-TOF analyses confirmed the ability of the adventitious roots of B. anthelmintica to produce rhamnetin. When the 15-day old root cultures were treated with 0.6 mM methyl jasmonate, on the 6th-day, rhamnetin content was enhanced to 0.443 mg g⁻¹DW, which was 1.5 folds compared to untreated roots. Similarly, Salicylic acid also improved rhamnetin production to 0.36 mg g⁻¹ DW, which was 1.2 folds increment compared to untreated roots. Rhamnetin content in the roots of field-grown plants was 0.112 mg g⁻¹DW.

Graphical Abstract

Keywords:

• Adventitious root culture
• IBA
• Rhamnetin
• Methyl Jasmonate
• Salicylic acid
• elicitation

Acknowledgment

DST FIST/India and DST-PURSE/India provided the instrumentation support for analyses.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Kerala State Biodiversity Board (KSBB) funded the project with grant number 838/A1/2016/KSBB.