A new flavonoid from the thorn of Gleditsia sinensis Lam

Abstract

A new flavonoid, (2′′′E,6′′′S)-4′′-(6-hydroxy-2,6-dimethylocta-2,7-dienoyl)-vitexin (1), and five known compounds (2–6) were isolated from the thorn of Gleditsia sinensis Lam. Their structures were determined by comprehensive and comparative spectroscopic analysis of NMR and MS data. The absolute configuration of the new compound was deduced by analysis of the experimental and calculated ¹³C NMR data. The protective effects against lipopolysaccharide (LPS)-induced apoptosis in normal rat kidney tubule epithelioid (NRK 52e) cells of the isolated compounds were investigated in vitro, whose results showed that compound 1 showed significant protective effect with the EC₅₀ value of 3.0 μM.

Graphical Abstract

Keywords:

• Gleditsia sinensis Lam.
• Fabaceae
• flavonoids
• renoprotective effect

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Central Leading Local Science and Technology Development Special Foundation ([2016]149), Henan Province High-level Personnel Special Support (ZYQR201810080), and Doctoral Research Funding of Henan University of Chinese Medicine (RSBSJJ2018-1).