Abstract
Ganoderma lucidum (G. lucidum) is a famous medicinal fungus used as a traditional medicine for generations in China. Among bioactivities that G. lucidum possesses, the anti-tumor effect has aroused extensive interests. In this study, one new triterpene (1) was isolated from 90% ethanol extract of the dried fruiting bodies of G. lucidum. Its structure was determined based on the analysis of its spectroscopic data, including 1 D and 2 D NMR and MS spectra. Furthermore, 1 elicited moderate anti-tumor activities (IC50 values of 15.38 ± 0.34 and 18.61 ± 0.55 μM for A549 and HepG2 cells, respectively) compared with cisplatin which was employed as the positive drug with IC50 values of 8.21 ± 0.17 and 5.36 ± 0.29 μM for A549 and HepG2 cells, respectively. The mechanism study by RT-qPCR and western blot analysis suggested that the p53/caspase-3 pathway is involved in the 1-induced apoptosis.
Graphical Abstract
Acknowledgement
The authors sincerely thank the support by Administration of Traditional Chinese Medicine of Guangdong Province [grant number 20211341] , National Natural Science Foundation of China [grant number 81803689], the Natural Science Foundation of Guangdong Province [grant number 2018A030310292], and the YIWEN Talent Project of The Third Affiliated Hospital of Guangzhou Medical University [grant number 2021#9].
Declaration of interest statement
The Authors Report No Declarations Of Interest.